At a glance
How it works
The skin model is based on a 3D reconstituted human epidermis which consists of human-derived keratinocytes that mimic the properties of in vivo skin, including a multi-layered stratum corneum. The principle behind the test method is that corrosive substance penetrates by diffusion or erosion and irreversibly damage the stratum corneum, which exposes the underlying cell layers to cytotoxicity from the substance.
Test items are directly applied to the skin tissue surface at the air-liquid interface, and skin corrosion is determined by measuring the cell viability with the MTT assay.
Corrosive compounds are classified as such if the test item decreases the cell viability below the defined thresholds levels (50% after 3 minutes or 15% after 60 minutes for UN GHS category 2) and as non-corrosive if the viability is greater than 50% after 3 minutes or 15% after 60 minutes.