In Vitro Skin Corrosion.

OECD TG 431: Reconstructed Human Epidermis Test Method

This in vitro skin corrosion test uses a Reconstructed Human Epidermis (RhE) model to distinguish between corrosive and non-corrosive substances, providing a clear binary hazard classification.

Fully accepted by regulatory authorities, the test is conducted in accordance with OECD Test Guideline 431 and supports classification in line with the UN Globally Harmonized System (GHS) for Classification and Labelling.

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Why testing with SenzaGen?

At a glance

Test system3D RECONSTRUCTED HUMAN EPIDERMIS.
ComplianceOECD TG 431.
What it measures VIABILITY OF SKIN CELLS.
ReadoutCORROSIVE OR NON-CORROSIVE (UN GHS CATERGORY 1).
Turnaround time6-8 weeks for standard studies.
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How it works

The test method is based on a 3D reconstituted human epidermis model which consists of human-derived keratinocytes that mimic the properties of in vivo skin, including a multi-layered stratum corneum. The principle behind the test method is that corrosive substance penetrates by diffusion or erosion and irreversibly damage the stratum corneum, which exposes the underlying cell layers to cytotoxicity from the substance.

Test items are directly applied to the skin tissue surface at the air-liquid interface, and skin corrosion is determined by measuring the cell viability with the MTT assay.

Corrosive compounds are classified as such if the test item decreases the cell viability below the defined thresholds levels (50% after 3 minutes or 15% after 60 minutes for UN GHS category 2) and as non-corrosive if the viability is greater than 50% after 3 minutes or 15% after 60 minutes.

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