An alternative biomarker-based approach for the prediction of proteins known to sensitize the respiratory tract.

Toxicol In Vitro. 2017 Oct 7;46:155-162. doi: 10.1016/j.tiv.2017.09.029.

Zeller KS, Johansson H, Lund TØ, Kristensen NN, Roggen EL, Lindstedt M.



Many natural and industrial proteins are known to have properties that can result in type I hypersensitivity, however, to date, no validated test system exists that can predict the sensitizing potential of these allergens. Thus, the objective of this study was to develop a protocol based on the myeloid cell-based Genomic Allergen Rapid Detection (GARD) assay that can be used to assess and predict the capacity of protein allergens known to induce sensitization in the respiratory tract. Cellular responses induced by eight selected proteins were assessed using transcriptional profiling, flow cytometry and multiplex cytokine analysis. 391 potential biomarkers were identified as a predictive signature and a series of cross-validations supported the validity of the model. These results together with biological pathway analysis of the transcriptomic data indicate that the investigated cell system is able to capture relevant events linked to type I hypersensitization.

Biomarker; Cell-based assay; Dendritic cells; Protein allergens; Respiratory sensitization

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