GARD®skin Dose-Response.

In vitro skin sensitization potency assessment

GARD®skin Dose-Response is an in vitro test assay designed to predict skin sensitization potency of chemicals.

The assay provides a Point-of-Departure (PoD) for risk assessment, enabling potency ranking and sub-classification of candidate ingredients. This makes it a valuable tool for both product development and regulatory decision-making.

By estimating the threshold concentration at which a test substance induces skin sensitization, the assay allows for potency assessment, where lower concentrations indicate higher potency. These data support the prediction of LLNA EC3 values, human skin sensitizing potency NOEL/NESIL, and UN GHS/CLP classification 1A or 1B, all with high statistical significance.

GARD®skin Dose-Response is adapted from GARD®skin (OECD TG 442E) and is currently included in the OECD Test Guideline Program (TGP 4.106).

196 genes

The number of biomarkers used in GARDskin Dose-Response.