Entries by Hanna Frykman

GARD – Genomic Allergen Rapid Detection. A Testing Strategy for Assessment of Chemical Sensitizers

Doctoral thesis defense date 2013-06-05 09:00     ISBN978-91-7473-519-2 Johansson, Henrik LU Abstract (Swedish) Popular Abstract in Swedish Vi lever i en tid och i ett samhälle där kemikalier har blivit en naturlig del av vår industrialiserade vardag. Till stor del ligger kemikalieindustrin bakom flera av de stora tekniska framsteg som gör att västvärlden har […]

The GARD assay for assessment of chemical skin sensitizers.

Toxicol In Vitro. 2013 Apr;27(3):1163-9. doi: 10.1016/j.tiv.2012.05.019. Epub 2012 Sep 29. Johansson H., Albrekt A.S., Borrebaeck C.A.K., Lindstedt M Abstract Allergic contact dermatitis is a skin disease caused by an immunologic reaction to low molecular weight compounds, so called haptens. These substances are commonly present in products used by humans in daily life, such as […]

Signature biomarker analysis for prediction of skin sensitizers using a cell-based in vitro alternative to animal experimentation

Henrik Johansson, Ann-Sofie Albrekt, Carl A.K. Borrebaeck, Malin Lindstedt Department of Immunotechnology, Lund University, BMC D13, 21184 Lund, Sweden Introduction Allergic contact dermatitis is an inflammatory skin disease caused by immunological responses towards chemical haptens. Current test of sensitizing chemicals rely on animal experimentation. New legislations on the registration and use of chemicals within pharmaceutical […]

Pattern rules: biomarker signatures for sensitization as an alternative to animal testing

Biomarkers in Medicine, December 2011 ,Vol. 5, No. 6 , Pages 809-811 (doi: 10.2217/bmm.11.82) Lindstedt M., Borrebaeck C.A.K. “Identification of relevant endpoints and biomarkers is essensial for development of alternative assays predicting sensitisation. Omics-based techniques have recently generated very promising biomarker patterns, as well as pathways, which will be used in the identification of sensitising agents” […]

A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests.

BMC Genomics. 2011 Aug 8;12:399. doi: 10.1186/1471-2164-12-399. Johansson H., Albrekt A.S., Lindstedt M., Borrebaeck C.A.K Abstract BACKGROUND: Allergic contact dermatitis is an inflammatory skin disease that affects a significant proportion of the population. This disease is caused by an adverse immune response towards chemical haptens, and leads to a substantial economic burden for society. Current […]

Functional and transcriptional profiling of MUTZ-3, a myeloid cell line acting as a model for dendritic cells

Immunology. 2006 Feb; 117(2): 156–166. doi:  10.1111/j.1365-2567.2005.02274.x Larsson K., Lindstedt M., Borrebaeck C.A.K. ABSTRACT The incidence of allergy is steadily increasing, but the molecular mechanisms involved in the allergic immune response are still not fully understood. In particular, further investigations focusing on dendritic cells, which are central in orchestrating the immune response, are needed. The […]