Extended solvent selection for in vitro sensitization testing using GARD®
Olivia Larne, Ulrika I Torstensdotter Mattson, Rikard Alm, and Gunilla Grundström.
SenzaGen, Lund, Sweden.
Introduction
The GARD®skin assay is an in vitro assay developed for the assessment of skin sensitizers. It is based on SenzaCells™, a human dendritic-like cell line, and a biomarker signature analyzed by a prediction model including pattern recognition and machine learning.
During the development of the GARD®skin platform, two solvents were used: DMSO (0.1%) and water. To increase the applicability domain of GARD®skin and the possibility to dissolve certain test items, for e.g. hard to dissolve substances and UVCBs, where show a broader range of solvents compatible with GARD®skin. Also, use of higher concentrations of the tested solvents were explored for the possibility to increase test item concentrations.
Concluding highlights
GARD®skin compatible solvents:
- Acetone
- DMF
- DMF/Glycerol
- DMSO
- Ethanol
- Glycerol
- Isopropanol
Increased applicability domain.
Poster presented at Eurotox, Helsinki, Sep 9, 2019.
